6MY0

Structure of 53BP1 Tandem Tudor domains with E1549P and D1550N mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	73.100, 141.270, 47.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.587 - 2.200
R-factor	0.192
Rwork	0.187
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g3r
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.587	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.087	0.553
Rmeas	0.091	0.580
Rpim	0.026	0.171
Number of reflections	12755	1078
<I/σ(I)>	23.43	5.3
Completeness [%]	98.6	87.36
Redundancy	12.2	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	1.5 M sodium/potassium phosphate, pH 6.0