Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.169, 65.169, 103.264 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.230 - 2.350 |
| R-factor | 0.21101 |
| Rwork | 0.208 |
| R-free | 0.26176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o09 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.742 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.00049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.068 | 0.923 |
| Number of reflections | 11073 | 1056 |
| <I/σ(I)> | 21.4 | 2.5 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 9.7 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | sodium phosphate, potassium phosphate |
