6MWY

The Prp8 intein of Cryptococcus gattii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	73.787, 73.787, 191.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.836 - 2.594
Rwork	0.220
R-free	0.27920
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.430	2.687
High resolution limit [Å]	2.594	2.594
Rmerge	0.050	0.826
Rmeas	0.052	0.860
Number of reflections	17152
<I/σ(I)>	44.49	4.28
Completeness [%]	99.7	97.43
Redundancy	13.6	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.5 M Sodium citrate, 0.1M Hepes, pH 7.5, 10 mM DTT, 2% isopropanol