6MWR

Recognition of MHC-like molecule

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	88.390, 88.390, 257.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.450 - 3.300
R-factor	0.239
Rwork	0.238
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gup 1hxm 4lfh
RMSD bond length	0.007
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.390	3.480
High resolution limit [Å]	3.300	3.300
Rpim	0.119	0.456
Number of reflections	16174	2287
<I/σ(I)>	6.7	2.2
Completeness [%]	99.9	100
Redundancy	8.5	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15-17% PEG3350 0.2M CaCl2