6MWA

NavAb Voltage-gated Sodium Channel, residues 1-239

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	124.772, 124.772, 189.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.884 - 2.400
R-factor	0.2149
Rwork	0.213
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.005
RMSD bond angle	0.687
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	28623
<I/σ(I)>	22.6
Completeness [%]	97.4
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	1.8 M Ammonium Sulfate 100mM Sodium Acetate pH 5.8