6MW8

UDP-galactose:glucoside-Skp1 alpha-D-galactosyltransferase with bound UDP and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	84.055, 84.055, 76.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.591 - 1.756
R-factor	0.1824
Rwork	0.181
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.784
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.13_2998))
Refinement software	PHENIX (0.8.6.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.591	37.591
High resolution limit [Å]	1.750	1.750
Number of reflections	28125
<I/σ(I)>	36.94
Completeness [%]	99.9
Redundancy	28.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	8-12% PEG4000, 0.4M ammonium sulfate, 0.1M sodium acetate