6MVV
NavAb voltage-gated sodium channel, I217C/F203A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 125.782, 125.969, 192.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.900 |
| R-factor | 0.223 |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rvy tetramer |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.834 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.138 | |
| Number of reflections | 68271 | |
| <I/σ(I)> | 24 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 | |
| CC(1/2) | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 1.8 M Ammonium Sulfate 100mM Sodium Acetate, pH 5.0 |
