6MVI

Apo Cel45A from Neurospora crassa OR74A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	APEX II CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	30.817, 72.975, 86.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.487 - 1.890
R-factor	0.1738
Rwork	0.167
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5glx
RMSD bond length	0.006
RMSD bond angle	0.831
Data reduction software	APEX 2
Data scaling software	APEX 2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.490	1.960
High resolution limit [Å]	1.890	1.890
Rmeas	0.207	0.993
Number of reflections	16371
<I/σ(I)>	6.6	1.2
Completeness [%]	98.3	95.9
Redundancy	7.4
CC(1/2)	0.987	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	100 mM HEPES pH 8.0, 20% PEG6000 (w/w) and 10 mM ZnCl2