6MVH
Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.417, 137.407, 108.833 |
| Unit cell angles | 90.00, 91.88, 90.00 |
Refinement procedure
| Resolution | 29.269 - 2.400 |
| R-factor | 0.2097 |
| Rwork | 0.209 |
| R-free | 0.26810 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.997 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.049 | 0.214 |
| Number of reflections | 106453 | 10707 |
| <I/σ(I)> | 14.04 | 3.87 |
| Completeness [%] | 98.3 | 99.07 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M calcium acetate, 50% PEG 8000, imidazole pH 8.0 |
