6MVG
Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 236.028, 127.078, 88.819 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 29.876 - 2.800 |
| R-factor | 0.1873 |
| Rwork | 0.185 |
| R-free | 0.24560 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.077 | 0.607 |
| Number of reflections | 63747 | 6333 |
| <I/σ(I)> | 14.03 | 2.36 |
| Completeness [%] | 99.7 | 99.91 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20.4 mg/mL protein, 0.13 M magnesium acetate, 10% PEG 8000 |
