6MV6

Crystal structure of RNAse 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-09
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	27.830, 38.780, 108.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.453 - 1.500
R-factor	0.1719
Rwork	0.169
R-free	0.22710
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.500	1.550
High resolution limit [Å]	1.500	1.500
Rmeas		0.283
Number of reflections	19372
<I/σ(I)>	14.5
Completeness [%]	99.1	98.9
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		280	Peg 4000, 50mM Sodium phosphate