6MUZ

Lysozyme, room temperature structure solved by serial 3 degree oscillation crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-03-03
Detector	DECTRIS EIGER X 1M
Wavelength(s)	1.2155
Spacegroup name	P 43 21 2
Unit cell lengths	79.100, 79.100, 38.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 1.839
R-factor	0.172
Rwork	0.170
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dpx
RMSD bond length	0.006
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.550	1.905
High resolution limit [Å]	1.839	1.839
Rmeas	0.339
Number of reflections	10841	981
<I/σ(I)>	10.01
Completeness [%]	98.9
Redundancy	10.01
CC(1/2)	0.991	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	277	50 mg/ml Lysozyme solution (in deionized H2O) was used for crystallization. A mixture of 1 mL of Lysozyme sample and 3 mL of precipitant (20% w/v NaCl, 6% PEG 6K, 0.5 M NaOAc pH 4.0)