6MUA
Crystal structure of Csm1-Csm4 subcomplex in the type III-A CRISPR-Csm interference complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.205100 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 156.433, 156.433, 187.275 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.440 - 2.910 |
| R-factor | 0.2314 |
| Rwork | 0.229 |
| R-free | 0.27772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mur |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.034 | 0.376 |
| Number of reflections | 30293 | 2973 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M phosphate-citrate pH 4.2, 5% PEG3000, 25% 1,2-propanediol, and 10% glycerol |
