6MU5

Bst DNA polymerase I TNA/DNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.390, 93.280, 103.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.839 - 1.912
R-factor	0.1918
Rwork	0.191
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l3s
RMSD bond length	0.007
RMSD bond angle	0.976
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.840	1.980
High resolution limit [Å]	1.910	1.910
Rmerge	0.718	2.130
Number of reflections	65358
<I/σ(I)>	41.14
Completeness [%]	98.7
Redundancy	19.7
CC(1/2)	0.821	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD