6MU0

Crystal Structure of Ribose-5-phosphate Isomerase B from Mycoplasma genitalium with bound Ribulose-5-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-16
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	45.350, 45.350, 138.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.099 - 1.100
R-factor	0.1346
Rwork	0.134
R-free	0.14870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3he8
RMSD bond length	0.005
RMSD bond angle	0.933
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX ((dev_3283))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.099	43.099	1.130
High resolution limit [Å]	1.100	4.920	1.100
Rmerge	0.111	0.096	0.518
Rmeas	0.118	0.103	0.559
Total number of observations	539804
Number of reflections	59627	797	4225
<I/σ(I)>	11.67	19.02	3.23
Completeness [%]	99.7	99.4	97.2
Redundancy	9.053	7.851	7.009
CC(1/2)	0.994	0.987	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	MygeA.00966.a.B1.PW38486 at 15.59 mg/ml was incubated with 5 mM ribose-5-phosphate, then was mixed 1:1 JCSG+(d5): 70% (v/v) MPD 0.1 M HEPES free acid, pH 7.0, Tray: 302126d5, puck: ppf0-7