6MTY

Crystal structure of a human anti-ZIKV-DENV neutralizing antibody MZ4 isolated following ZPIV vaccination

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	60.358, 67.582, 138.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.977 - 2.951
R-factor	0.2185
Rwork	0.217
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uta
RMSD bond length	0.003
RMSD bond angle	0.704
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.120
High resolution limit [Å]	2.950	2.950
Rpim	0.120	0.600
Number of reflections	11975	1055
<I/σ(I)>	7.4
Completeness [%]	94.4
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2 M ammonium sulfate, 0.1 M HEPES (pH 7.5), 25% (w/v) polyethylene glycol 3,350