6MTS

Crystal structure of VRC43.03 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	67.424, 69.628, 122.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.923 - 2.437
R-factor	0.2447
Rwork	0.242
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mtr VRC43.01
RMSD bond length	0.002
RMSD bond angle	0.664
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.410	2.410
Rmerge	0.154	0.405
Rpim	0.086	0.280
Number of reflections	18236
<I/σ(I)>	9.3
Completeness [%]	83.2
Redundancy	3.7
CC(1/2)	0.899	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	40% PEG 400, 8% PEG8K, 0.1M HEPES 7.4