6MTR
Crystal structure of VRC43.01 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.075, 54.661, 66.113 |
| Unit cell angles | 90.00, 113.30, 90.00 |
Refinement procedure
| Resolution | 36.863 - 1.798 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model of VRC43.01 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.798 | 1.800 |
| Rmerge | 0.111 | 0.733 |
| Rpim | 0.067 | 0.440 |
| Number of reflections | 42070 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 84.8 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.883 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 34% PEG 4000, 0.2M Ammonium Acetate, 0.1M Na Citrate 5.6 |
