6MTQ
Crystal structure of VRC42.N1 Fab in complex with T117-F MPER scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.206, 70.257, 212.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.847 - 2.700 |
R-factor | 0.2088 |
Rwork | 0.206 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mtp VRC42.04 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.492 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.145 | 0.717 |
Rpim | 0.099 | 0.499 |
Number of reflections | 18549 | |
<I/σ(I)> | 9.3 | |
Completeness [%] | 97.3 | |
Redundancy | 2.9 | |
CC(1/2) | 0.864 | 0.521 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 5% PEG 400, 0.1M Magnesium sulfate, 2M Ammonium sulfate, 0.1M Tris, pH 8.5 |