6MT7

Phlebotomus duboscqi salivary D7 protein, selenomethionine derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97499
Spacegroup name	P 21 21 2
Unit cell lengths	143.908, 101.515, 33.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.371 - 1.780
R-factor	0.1702
Rwork	0.168
R-free	0.20620
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	1.810
High resolution limit [Å]	1.780	4.830	1.780
Rmerge	0.065	0.043	0.194
Rmeas	0.074	0.049	0.229
Rpim	0.033	0.022	0.118
Number of reflections	90692	4540	4486
<I/σ(I)>	12.4
Completeness [%]	99.7	99.9	98.7
Redundancy	4.5	4.8	3.4
CC(1/2)		0.998	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 6000m 0.1M MES pH 6