6MSX
Iron containing ferritin at 1.43A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9202 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.590, 181.590, 181.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.430 |
| R-factor | 0.15792 |
| Rwork | 0.157 |
| R-free | 0.17170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ier |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.646 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Number of reflections | 45041 | 3445 |
| <I/σ(I)> | 31.81 | |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 138.2 | 48.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | A 20 uL pellet of 50 mM cadmium sulfate in 2% agar was deposited on a cover slip. Adjacent to this was positioned ferritin (10 ul of 50 mg/ml ferritin, Sigma F4503) and precipitant (10 ul of 3.5M Ammonium Sulfate and 100 mM Tris pH 7.4). The cover slip was equilibrated over 3.5 M Ammonium Sulfate |
