6MSV
Structure of the 6th type III domain from human fibronectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.520, 79.460, 80.886 |
| Unit cell angles | 112.33, 95.88, 94.48 |
Refinement procedure
| Resolution | 26.640 - 2.400 |
| R-factor | 0.2379 |
| Rwork | 0.235 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dft |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.702 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.640 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 46905 | 4387 |
| <I/σ(I)> | 13.1 | 1.6 |
| Completeness [%] | 90.2 | 78.1 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M Ammonium Sulfate |
