6MSI

TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-11-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	33.157, 40.038, 44.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 1.650
R-factor	0.215 *
R-free	0.28000 *
Structure solution method	ISOMORPHOUS
Starting model (for MR)	1msi
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.067 *
Total number of observations	26882 *
Number of reflections	7041 *
<I/σ(I)>	20.96	3.53
Completeness [%]	92.5	89.3
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	20 *	Jia, Z.,(1995) Protein Sci., 4, 1236. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3-3.5 (mg/ml)
2	1	drop	ammonium sulfate	25-27.5 (%sat)
3	1	drop	sodium acetate	0.05 (M)
4	1	reservoir	ammonium sulfate	50-55 (%sat)
5	1	reservoir	sodium acetate	0.1 (M)