6MS8
Crystal Structure of Chalcone Isomerase from Medicago Truncatula Complexed with (2S) Naringenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 85.090, 85.090, 221.508 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.351 - 1.900 |
| R-factor | 0.2295 |
| Rwork | 0.228 |
| R-free | 0.24070 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.690 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.300 |
| Rpim | 0.300 | |
| Number of reflections | 139011 | 6378 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 98.3 | 90.6 |
| Redundancy | 4.8 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 30% (v/v) PEG1K, 100 mM Tris (pH 8.5) and 50 mM sodium malonate |
