6MRX
Sialidase26 apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99999 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 115.476, 79.817, 260.811 |
| Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
| Resolution | 48.420 - 2.000 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q6k |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.591 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.420 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 157373 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 20% PEG 6000, 0.1 M Tris-HCl pH 8.0 |
