6MRV
Sialidase26 co-crystallized with DANA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99995 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 92.040, 116.700, 113.636 |
| Unit cell angles | 90.00, 106.34, 90.00 |
Refinement procedure
| Resolution | 60.780 - 1.800 |
| R-factor | 0.2036 |
| Rwork | 0.202 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q6k |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.780 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.780 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 106390 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 20% PEG 6000, 0.1 M Tris-HCl pH 8.0 |
