6MRN
Crystal Structure of ChlaDUB2 DUB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-11-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.363, 74.372, 77.407 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.630 - 2.290 |
| R-factor | 0.19828 |
| Rwork | 0.196 |
| R-free | 0.24725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hag |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.630 | 2.400 |
| High resolution limit [Å] | 2.290 | 2.340 |
| Rmerge | 0.181 | |
| Rmeas | 0.200 | |
| Rpim | 0.107 | |
| Number of reflections | 10827 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES sodium pH 7.5, 2% (v/v) PEG 400, 2M ammonium sulfate, 0.3M NDSB-195 |
