6MRK
Crystal structure of dmNxf2 NTF2-like domain in complex with Nxt1/p15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.540, 74.090, 155.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.910 - 2.800 |
| R-factor | 0.21705 |
| Rwork | 0.212 |
| R-free | 0.26259 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jkg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.565 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.900 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.102 | 0.412 |
| Number of reflections | 19083 | 2732 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 5.3 |
| CC(1/2) | 0.995 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 1.6 M magnesium sulfate |
