6MR3
Crystal structure of the competence-damaged protein (CinA) superfamily protein from Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.030, 54.507, 111.013 |
| Unit cell angles | 90.00, 125.38, 90.00 |
Refinement procedure
| Resolution | 41.212 - 2.050 |
| R-factor | 0.1868 |
| Rwork | 0.186 |
| R-free | 0.21520 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.777 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 54303 | 2619 |
| <I/σ(I)> | 13.49 | 3.41 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M bis-tris ph5.5, 0.2M ammonium acetate, 25%PEG3350, papain protease |
