6MQO
Structure of HIV-1 CA G208R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979200 |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.970, 91.970, 57.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.20903 |
| Rwork | 0.206 |
| R-free | 0.27132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xfx |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.849 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.095 | |
| Number of reflections | 4626 | 1671 |
| <I/σ(I)> | 9.31 | 1.67 |
| Completeness [%] | 97.4 | 94.7 |
| Redundancy | 2.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 15% PEG3350, 0.1 M sodium iodide, Bis-Tris propane, pH 6.5 |
