6MPS
TagT bound to LIIa-WTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.373, 66.373, 145.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.355 - 1.860 |
| R-factor | 0.2165 |
| Rwork | 0.216 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mej |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.551 |
| Data reduction software | XDS (May 1, 2016) |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.390 | 60.390 | 1.900 |
| High resolution limit [Å] | 1.860 | 9.090 | 1.860 |
| Rmerge | 0.057 | 0.019 | 1.882 |
| Rmeas | 0.065 | 0.023 | 2.150 |
| Rpim | 0.031 | 0.012 | 1.023 |
| Total number of observations | 118106 | ||
| Number of reflections | 27947 | 280 | 1591 |
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 99.2 | 94.2 | 94.4 |
| Redundancy | 4.2 | 3.6 | 4.2 |
| CC(1/2) | 0.999 | 0.999 | 0.304 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM HEPES pH 7.0-7.5, 20-22% w/v PEG 3350, and 200 mM MgCl2 |
