6MPN
Racemic M2-TM I42E crystallized from racemic detergent
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P -1 |
Unit cell lengths | 14.680, 37.250, 43.220 |
Unit cell angles | 107.41, 96.73, 97.66 |
Refinement procedure
Resolution | 18.335 - 1.400 |
R-factor | 0.2306 |
Rwork | 0.230 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.335 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmeas | 0.077 | 0.751 |
Rpim | 0.039 | 0.390 |
Number of reflections | 14625 | 746 |
<I/σ(I)> | 10.08 | 1.81 |
Completeness [%] | 86.8 | 43.45 |
Redundancy | 3.8 | 3.6 |
CC(1/2) | 0.998 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.8 M imidazole |