6MPM

Racemic M2-TM I42A crystallized from racemic detergent

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	.97856
Spacegroup name	P -1
Unit cell lengths	17.290, 18.530, 40.240
Unit cell angles	81.16, 87.97, 69.07

Refinement procedure

Resolution	17.109 - 1.400
R-factor	0.2215
Rwork	0.221
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.109	1.450
High resolution limit [Å]	1.400	1.400
Rmeas	0.058	0.728
Rpim	0.030	0.373
Number of reflections	8606	892
<I/σ(I)>	10.93	2.27
Completeness [%]	94.6	94.39
Redundancy	3.8	3.8
CC(1/2)	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1 M Tris-HCl pH 8.5 0.1 M sodium citrate tribasic 5% v/v PEG400