6MP3

Binary structure of DNA polymerase eta in complex with templating hypoxanthine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.650, 98.650, 81.242
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.717 - 1.907
R-factor	0.1703
Rwork	0.168
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.940
High resolution limit [Å]	1.910	5.180	1.910
Rmerge	0.119	0.052	0.996
Rmeas	0.126	0.055	1.053
Rpim	0.040	0.017	0.341
Total number of observations	350721
Number of reflections	35061	1816	1736
<I/σ(I)>	4.3
Completeness [%]	100.0	100	99.9
Redundancy	10	10.2	9.4
CC(1/2)		0.998	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	100mM MES 5.5, PEG 2K MME, 10mM MgSo4