6MOV
Crystal Structure of the All-trans Retinal bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121Q Human Cellular Retinoic Acid Binding Protein II in the Dark at 1.75 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-15 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.219, 59.219, 99.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.618 - 1.752 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.618 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.055 | |
| Rmeas | 0.074 | 0.974 |
| Number of reflections | 20945 | 2022 |
| <I/σ(I)> | 45.06 | 2.4 |
| Completeness [%] | 100.0 | 98.92 |
| Redundancy | 10.9 | 11 |
| CC(1/2) | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, DL-malic acid |
