6MOT

Bacteroides intestinalis feruloyl esterase, Bacint_01033

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-07
Detector	RAYONIX MX300HE
Wavelength(s)	0.979
Spacegroup name	P 64 2 2
Unit cell lengths	99.959, 99.959, 164.204
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	86.567 - 1.710
R-factor	0.1713
Rwork	0.171
R-free	0.19120
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	xia2
Phasing software	SOLVE
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.567	1.730
High resolution limit [Å]	1.710	1.710
Number of reflections	99242
<I/σ(I)>	19.3
Completeness [%]	100.0
Redundancy	40.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	1.5M Lithium sulfate, 0.1 M sodium acetate (Hampton SaltRx B10)