6MOR
Crystal Structure of the All-Trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121Y Mutant of Human Cellular Retinoic Acid Binding Protein II in the Dark at 1.79 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-12 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97856 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.469, 58.469, 100.118 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.185 - 1.790 |
R-factor | 0.2248 |
Rwork | 0.223 |
R-free | 0.24720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.007 |
RMSD bond angle | 0.944 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.185 | 1.820 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.044 | 0.509 |
Rmeas | 0.063 | 0.656 |
Number of reflections | 19270 | 1890 |
<I/σ(I)> | 39.94 | |
Completeness [%] | 100.0 | 99.89 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | PEG 3350, Sodium Malonate |