6MOG
Dimeric DARPin C_R3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.250, 89.810, 44.270 |
| Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
| Resolution | 33.248 - 1.210 |
| R-factor | 0.1418 |
| Rwork | 0.141 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | predicted model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS (Jun 1, 2017) |
| Data scaling software | XSCALE (Jun 1, 2017) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.250 | 1.253 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.051 | 1.564 |
| Rpim | 0.021 | 0.643 |
| Number of reflections | 83274 | 8248 |
| <I/σ(I)> | 14.8 | 1.34 |
| Completeness [%] | 88.2 | 72 |
| Redundancy | 6.8 | 6.7 |
| CC(1/2) | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris pH 8.5, 25% PEG 3350, 1 mM reduced glutathione, 1 mM oxidized glutathione, 25% ethylene glycol cryoprotectant |
