6MNZ

Crystal structure of RibBX, a two domain 3,4-dihydroxy-2-butanone 4-phosphate synthase from A. baumannii.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-11-02
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	116.707, 136.857, 51.856
Unit cell angles	90.00, 98.53, 90.00

Refinement procedure

Resolution	68.430 - 2.660
R-factor	0.2791
Rwork	0.278
R-free	0.30300
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.430	2.700
High resolution limit [Å]	2.660	2.660
Rmerge	0.131	1.122
Rmeas	0.153	1.309
Rpim	0.079	0.672
Number of reflections	22952	1147
<I/σ(I)>	7.4	1.2
Completeness [%]	99.1	99.7
Redundancy	3.7	3.8
CC(1/2)	0.993	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10 mM MES ph 6.5, 100 mM NaCl, 150 mM ammonia sulfate, 19% (w/v) PEG 1000