6MNQ
Rhesus macaque anti-HIV V3 antibody DH727.2 with gp120 V3 ZAM18 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2017-06-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.511, 95.780, 58.049 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 28.993 - 1.804 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 4HCR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.067 | 0.401 |
| Number of reflections | 41285 | 2038 |
| <I/σ(I)> | 16.7 | 3.2 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 20% PEG 6,000 |
