6MNJ

Hadza microbial sialidase Hz136

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99994
Spacegroup name	I 2 2 2
Unit cell lengths	119.670, 145.395, 150.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.700 - 2.200
R-factor	0.2148
Rwork	0.213
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q6k
RMSD bond length	0.002
RMSD bond angle	0.593
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.140	2.250
High resolution limit [Å]	2.200	2.200
Number of reflections	128523
<I/σ(I)>	10.5
Completeness [%]	100.0
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20% PEG 3350, 0.1 M Bis Tris Propane, 0.2 M Sodium Citrate