6MMQ

Carbon regulatory PII-like protein SbtB from Cyanobium sp. 7001 bound to cAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	46.649, 50.211, 66.561
Unit cell angles	97.60, 102.85, 102.49

Refinement procedure

Resolution	42.472 - 2.020
R-factor	0.2214
Rwork	0.218
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.005
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.480	2.070
High resolution limit [Å]	2.020	2.020
Number of reflections	35671
<I/σ(I)>	6.4
Completeness [%]	96.4
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Potassium Acetate, 16 % PEG 3350