6MMO

Carbon regulatory PII-like protein SbtB from Cyanobium sp. 7001 bound to AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	46.737, 51.332, 66.803
Unit cell angles	97.91, 102.55, 103.09

Refinement procedure

Resolution	43.221 - 1.860
R-factor	0.2121
Rwork	0.210
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.231
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.220	1.900
High resolution limit [Å]	1.860	1.860
Number of reflections	47208
<I/σ(I)>	9.1
Completeness [%]	97.1
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Sodium Acetate 18% PEG 3350