6MM5
Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 peptide (2799-2810)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.861, 74.881, 98.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.950 - 1.950 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o7l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.428 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.010 |
| High resolution limit [Å] | 1.950 | 4.460 | 1.950 |
| Rmerge | 0.056 | 0.036 | 0.661 |
| Rmeas | 0.063 | 0.040 | 0.740 |
| Rpim | 0.027 | 0.018 | 0.325 |
| Total number of observations | 157735 | ||
| Number of reflections | 30237 | 2697 | 2408 |
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 99.5 | 99.1 | 97.5 |
| Redundancy | 5.2 | 5 | 4.6 |
| CC(1/2) | 0.998 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M trimethylamine N-oxide, 0.1 M tris-HCl, 20% (w/v) PEG monomethyl ether 2K 25% (v/V) ethylene glycol |
