6ML7
ZBTB24 Zinc Fingers 4-8 with 19+1mer DNA Oligonucleotide (Sequence 4 with a CpG 5mC Modification)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-08 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.130, 68.707, 48.448 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 28.014 - 1.750 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ml6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.777 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.014 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.098 | |
| Rpim | 0.048 | 0.818 |
| Number of reflections | 30550 | 3045 |
| <I/σ(I)> | 8.4 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.8 | 2.9 |
| CC(1/2) | 0.426 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 30% PEG2000 MME, 0.1 M potassium thiocyanate |
