6ML6
ZBTB24 Zinc Fingers 4-8 with 19+1mer DNA Oligonucleotide (Sequence 4 with a CpA 5mC Modification)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-07 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.163, 68.244, 49.259 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 28.038 - 1.540 |
| R-factor | 0.2023 |
| Rwork | 0.202 |
| R-free | 0.22150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ml5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.538 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.040 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.074 | |
| Rpim | 0.036 | 0.724 |
| Number of reflections | 45159 | 4490 |
| <I/σ(I)> | 9.6 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.7 | |
| CC(1/2) | 0.392 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 25% PEG3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M ammonium acetate |
