6ML2

ZBTB24 Zinc Fingers 4-8 with 19+1mer DNA Oligonucleotide (Sequence 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	46.065, 68.619, 49.477
Unit cell angles	90.00, 91.90, 90.00

Refinement procedure

Resolution	38.232 - 1.874
R-factor	0.2523
Rwork	0.251
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5V3G (zinc fingers) + 18-mer B-DNA model
RMSD bond length	0.005
RMSD bond angle	0.644
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.120	1.940
High resolution limit [Å]	1.870	1.870
Rmerge	0.056	0.564
Rpim	0.024	0.264
Number of reflections	36754	699
<I/σ(I)>	22.6	2.1
Completeness [%]	77.7	27.6
Redundancy	5.6	3.9
CC(1/2)		0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	25% PEG3350, 0.1 M Bis-Tris, pH 6.5