6MKY

Human SDS22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	75.104, 76.546, 128.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.552 - 2.900
R-factor	0.2412
Rwork	0.238
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4aw4
RMSD bond length	0.002
RMSD bond angle	0.458
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.069	2.592
Number of reflections	16949	2677
<I/σ(I)>	10.6	2.2
Completeness [%]	99.6	99.5
Redundancy	7.3	7.4
CC(1/2)	0.965	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Ammonium sulfate, 0.01 M Cadmium chloride hemi(pentahydrate), 0.1 M HEPES 7.5, 25 % v/v PEG Smear Medium (12.5%w/v PEG 3350 12.5%w/v PEG 4000 12.5%w/v PEG 2000 12.5%w/v PEG 5000 MME)