6MKT
Photoactive Yellow Protein with 3-chlorotyrosine substituted at position 42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-11-20 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 1.18076 |
| Spacegroup name | P 63 |
| Unit cell lengths | 65.500, 65.500, 40.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.896 - 1.600 |
| R-factor | 0.1675 |
| Rwork | 0.167 |
| R-free | 0.18880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nwz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.880 |
| Data reduction software | MOSFLM |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.075 | |
| Number of reflections | 13140 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 77.5 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1M sodium chloride, 2.4M ammonium sulfate, pH = 6.0, drops were microseeded |
