6MJL

Crystal structure of ChREBP NLS peptide bound to importin alpha.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	120
Detector technology	CCD
Collection date	2006-04-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	77.434, 89.691, 96.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.434 - 2.500
R-factor	0.1999
Rwork	0.198
R-free	0.24440
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	23621
<I/σ(I)>	13.2
Completeness [%]	97.6
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	500 mM Na Citrate 10 mM DTT 20 mM HEPES pH 6.5